A simple antigen-antibody reaction using ultrasmall FeCo nanoparticles
نویسندگان
چکیده
In this study, we prepared ultrasmall FeCo nanoparticles (NPs) with a high magnetic moment and examined their antigen-antibody reaction for biodetection applications. The NPs were collected from the FeCo-BaF2 nanogranular film Fe:Co:Ba:F = 14:11:21:54 at.%, by dissolving in water since BaF2 matrix was deliquescent. size of ∼5 nm saturation magnetization estimated to be ∼15.30 kG (149.0 emu/g). Candida albicans antibodies (abcam ab53891)-conjugated using an ultracentrifugal separation (110 000 rpm, 90 min), they then reacted albicans. obtained result indicates that absorbed successfully onto NPs, number bound counted micrographs aggregates increased significantly adding sonication treatment before binding them antibodies. success improves detection sensitivity as well offers potential smaller biomolecules.
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ژورنال
عنوان ژورنال: AIP Advances
سال: 2023
ISSN: ['2158-3226']
DOI: https://doi.org/10.1063/9.0000569